Journal: Scientific Reports
Article Title: Aluminum adjuvants differentially induce IL-1β release in vitro yet share NLRP3 inflammasome-independent adjuvant effects in vivo
doi: 10.1038/s41598-025-34660-6
Figure Lengend Snippet: The antibody response to protein antigens injected with aluminum adjuvants does not require functional NLRP3. ( A , B ) C57BL/6J WT and Nlrp3 -/- mice were injected twice with OVA and AH ( A ) or nucleoprotein of SARS-CoV-2 (NP) and AP ( B ). The graphs show the mean ± SE of four mice per group. ( C , D ) CD-1 mice were treated with MCC950 or untreated and then injected twice with OVA and AH ( C ) or nucleoprotein of SARS-CoV-2 (NP) and AP ( D ). The graphs show data from individual mice and the mean ± SE of 4–5 mice per group. ns – not significant (Student’s t-test, p > 0.05).
Article Snippet: Endotoxin-free ovalbumin (OVA) was purchased from InvivoGen and the SARS-CoV-2 Nucleocapsid protein (NP) was purchased from Acro Biosystems (Newark, DE).
Techniques: Injection, Functional Assay